Many biologists find this a convenient way to rapidly check on the health of tissue cultures. Acid production: 1. 2. [13], Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. Phenol red exists as a red crystal that is stable in air. Find chemicals for your experiments at Home Science Tools! Polio (IPV – Ipol) Next. At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−. It is a weak acid with pK a = 8.00 at 20 °C (68 °F). endstream endobj startxref Material Safety Data Sheet or SDS for Phenol red 107241 from MilliporeSigma for download or viewing in the browser. [1] It is a weak acid with pKa = 8.00 at 20 °C (68 °F). Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. For example, a culture of relatively slowly dividing mammalian cells can be quickly overgrown by bacterial contamination. [1][7] However, this cyclic structure could not be confirmed by X-ray crystallography. 3 The two forms of the dye have different optical absorption spectra. Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories. Standardized for colorimetric pH determination. In Vitro ADMET Laboratories, LLC. [9] It was the first test of kidney function and was used for almost a century but is now obsolete. Phenol Red Indicator Solution. Albert P Li. This usually results in an acidification of the medium, turning it yellow. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. Specification Test Results. Chemical Formula: C 19 H 14 O 5 S Brand: OXFORD Previous. Hence, the relative concentration of one form, which varies as a function of … Phenol Red Indicator Solution. For example, phenol red exhibits an orange color between pH 6.8 and pH 8.4. �]���{̧$�S"�X�)�S�L`ͥt����2�k0H9N���uE��$�Rt� u��b �-�R�q I�EA�rwL��5� �@c��Q����L8��hY�r���ɱ-�H��#s�1=c1 ��X�ao1�5���d)�@�v��ɣ���<9��������s��ɣ�(���O�������G�]��2��jϿ������+�J�ļ�O���Z� This observed color change is because phenol red loses protons (and changes color) as the pH increases. Color range: pH 6.0-8.5. Standardized for colorimetric pH determination. MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. KS-00000XAO. This inexpensive indicator can be used to distinguish between acidic and basic solutions. [6], In several sources, the structure of phenol red is shown with the sulfur atom being part of a cyclic group, similar to the structure of phenolphthalein. If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. 59 0 obj <>/Filter/FlateDecode/ID[<460C89CDBDC0009432C14715F39BAA36><2BA3B43A4554254491AFA26C4B8FE729>]/Index[13 114]/Info 12 0 R/Length 172/Prev 212768/Root 14 0 R/Size 127/Type/XRef/W[1 3 1]>>stream Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. 126 0 obj <>stream (A table of other common chemical indicators is available in the article on pH indicators.). The phenol red dye indicator is a weak organic acid, and its unionized acid and base forms are present in a concentration ratio that is determined by the ionization constant of the acid and the pH of the medium according to the familiar Henderson-Hasselbach equation. SCHEMBL222482. Cite. h�b``�a``�b 'P�0p4p ��@1C � �(��u pH Indicator Ranges (Ascending pH) Acid-base pH indicators are solutions that have a characteristic color at certain pH levels. ,"]���n����S��^���`�*Xv*� ��`[5�f��U� q �[����-���Y�d�L�]��@�I9H��?�� 2s�&00��L�g`ڵ � �� 13 0 obj <> endobj When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. A solution of phenol red is used as a pH indicator: its color PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. DTXSID2044420. Add all three to Cart Add all three to List. Phenol red is yellow at a pH < 6.8 and red at a pH of > 7.4, therefore if a bacterium ferments a sugar to acid a yellow color will develop. High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). Phenol Red, sodium salt, pure, water soluble, indicator. Phenol red is frequently used in cell culture media as a pH indicator. The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used. h�bbd```b``>"@$S�T� "Y�"���`�"0��^ �"�R@$W&X6,k fk�H�H)} Phenol red is an indicator commonly used to measure pH in swimming pool test kits, see e.g. grade. Thermo Scientific Remel Phenol Red Solution is a pH indicator for use with microbiological culture media. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Phenol red is an acid-base indicator. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. Phenol Red sodium salt, powder. [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. 16th Jan, 2015. Above pH 8.2, phenol red turns a bright pink (fuchsia) color. In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color. Phenol Red sodium salt, ACS reagent. Typically, 15 mg/l are used for cell culture. Shop a large selection of Benzofurans products and learn more about Phenol Red, Pure, Indicator Grade, Fisher Chemical™: Benzofurans Organoheterocyclic compounds . grade. A solution of phenol red is used as a pH indicator: its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. DSSTox_GSID_44420. %%EOF Color range: pH 6.0-8.5. ���ɨ#�3#P[ƶ�.K�-CV�3v$N$p B�@�] �e8: �yP;P��C�Q?y��P�1 ����o�z�iЯ.����y�`�,�8{�m-b��yR!�7�� (�$ˁb9�� b�1���A�}(�#L���G�3P2�����2���~y�Yv�Ғ�w���HE�d�^����{L!�&r˜�A�0�KB��w�IF �9�KC���+ `/��w�"V)��N�؁ AvHv��j��.ĺ��45��g[/y���-�˽����z�`��#�*�{�r��c�n��ѐ v0&� ,a+Q��\� �B�p`�h��X`�8xk�G6�+&�M���h � ���ܬ��'�y� ����@b7��hYG��ؠ��,h��mR��m3�2@ύ��� ��@��Ɓ!�E�@�� �-���($`�9#@�pM�M�*�\�4��`�R����. They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. Calculations like this one might make a good project for a college freshman chemistry class. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. In addition, the waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow. Additionally, phenol red tubes contain an inverted Durham tube. Its color exhibits a gradual transition from yellow (λmax = 443 nm[2]) to red (λmax = 570 nm[3]) over the pH range 6.8 to 8.2. Gas Production 1. Phenol red is a pH indicator frequently used in cell biology laboratories. endstream endobj 14 0 obj <> endobj 15 0 obj <>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Rotate 0/TrimBox[0 0 595.275 841.889]/Type/Page>> endobj 16 0 obj <>stream use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue–culture system 1 2 �g���_p�����zq���D_�2������{�������볷W�W�}~������W�~����˿�}~���_~�8{�⮗����خ��O�8�� @N���/O����΀���=����?�������Y �:��l��ǧo�/~����o�g�. Other common chemical indicators is available in the absence of thiosulfate to inhibit the oxidizing.! Indicator frequently used in cell culture applications about phenol red added to this growth medium will have characteristic. By X-ray crystallography salt is used as a red crystal that is, a pH indicator which turns media. 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